Diatherix Laboratories utilizes its proprietary technology,
Diatherix founder Dennis Grimaud became involved with
Diatherix Laboratories was awarded the Technology Innovation Award for the first commercially available H1N1-09 test performed in a CLIA laboratory
Podiatry Today listed
The process of performing
Nucleic acid extraction methods used in
Amplification begins by adding the panel-specific Diatherix primer mixes (shown below) to the extracted nucleic acids (template) in a new 96-well plate. After the primers have been added to the template, the plate is then sealed and placed in an instrument for thermocycling. Each sample goes through customized cycles of heating and cooling to cause the PCR reactions to take place, replicating the targeted sequences of DNA for detection. To learn more about basic PCR visit this website or watch this animation .
The key to making
Nested target-specific primers are used during the initial target enrichment step.
A pair of SuperPrimers are then applied to amplify all targets.
The detection phase of the process is accomplished by measuring the fluorescence of special tags that are bound to the target sequences, which have been attached during amplification. For detection, each target sequence has a complementary sequence, or probe, bound to a specific location on an array plate(chemically-modified solid surface). Tagged PCR products are added to the array plate and undergo hybridization, a step in which DNA fragments will anneal to their specific, bound, complementary sequences. Unbound nucleic acids and reagents are then washed away. Lastly, the array is illuminated within a highly calibrated light reading instrument, which measures and records the RFUs (Relative Fluorescence Units) present for each target. If the RFUs are above the threshold for detection, then the target DNA is reported to be detected within the specimen.